AJPHR

American Journal Of Pharmacy And Health Research

ISSN NO.: 2321-3647
DOI: 10.21276/ajphr
February 2018 Issue 2
1

Comparison of Outcomes of Two Different Treatment Methods In The Management of Diabetic Foot Ulcer

M.V.R. Harsha Vardhan1, Y. Suraj Varma1, S. Parimalakrishnan1, Junior Sundresh*2

1.Department Of Pharmacy, Annamalai University

2.Deputy Medical Superintendent, Rajah Muthiah Medical College & Hospital, Annamalai University

ABSTRACT

The aim of this study was to compare the outcomes of surgical versus non- surgical treatment in the management of Diabetic Foot Ulcer. A Prospective Observational Study which was carried out in the Department of Surgery of Rajah Muthiah Medical College Hospital a tertiary health care hospital in Chidambaram, Cuddalore District, Tamilnadu in the period of November 2016 to April, 2017 (6 months). The patient demographic, disease, ulcer size, drugs and treatment prescribed were collected in a specially designed data collection form which also included bacterial culture tests. Upon enrolment for the study 65% (39) of the patients diagnosed for diabetic foot were male and 35% (21) were female. The demographic classification suggests that diabetic foot incidence has increased upon ages of people ranging between the mean ages of 45+/- 5 years to 60+/-5 years. The bacterial distribution over the patients showed that, out of which Escherichia coli (28.3%) and Staphylococcus aureus (15%) are the most common bacteria. The outcomes mainly depend upon the grading of infection and ulcer size. The superficial ulcers can be treated more effectively with antibiotics and regular dressings, whereas the deep ulcers and gangrene should be intervened with surgical procedures. The assessed study reports that clinical pharmacist intervention by patient education and medication counselling creates a significant influence in medication adherence

Keywords: Diabetic foot ulcer, surgical treatment, Non-surgical treatment, management

2

Inhibition of Formation of Fructose Induced Advanced Glycation End Products by Momordica chochinchinensis

Swathi Putta*, Eswar Kumar Kilari

Pharmacology Division, A.U. College of Pharmaceutical Sciences,  Andhra University, Visakhapatnam.

ABSTRACT

The present study evaluates the antioxidant potential of hydro alcoholic pericarp extract of Momordica chochinchinensis (HAPMC) using different model systems. To establish the link between glycation and oxidation processes, the HAPMC extract has been evaluated for its in vitro radical scavenging  activity against superoxide, hydroxyl, hydrogen peroxide, nitric oxide, ferric and ABTS+ radical and antiglycation activities like the inhibitory activities on bovine serum albumin (BSA) and as well as protein oxidation markers including protein carbonyl formation (PCO). The results indicated that the HAPMC possesses the highest antioxidant potential against superoxide, hydroxyl, hydrogen peroxide, nitric oxide, ferric radical and ABTS+ radical. The HAPMC extract at different concentrations (10–250 µg/ml) has significantly quenched the fluorescence intensity of glycated BSA and the glycoxidation measured in terms of advanced glycation end products (AGEs). Furthermore, the study demonstrated that the inhibitory effect of HAPMC extract in preventing oxidative protein damages including effect on PCO formation, which are believed to form under the glycoxidation processes. These results clearly demonstrate that the HAPMC, owning to its antioxidant content, is capable of suppressing the oxidative stress, carbonyl stress and formation of AGEs and protein oxidation in vitro.

Keywords: Advanced glycated end products, Oxidation, Momordica chochinchinensis

3

Social Anxiety and Problematic Internet Use among University Students

Rabina Debbarma*, G. Umadevi**

Department of Psychology, Annamalai University.

ABSTRACT

This study was conducted to find out the relationship between social anxiety and problematic internet use among university students. It was also tried to find out the difference between demographic variables like gender and locality. 200 university students were selected from Tripura University, in Tripura State through simple random sampling technique. Problematic Internet Use Questionnaire (Tatcher & Goolam, 2005) and Social Anxiety Questionnaire for Adults (SAQ-A30) developed by Callallo, Salazar, Irurtia, Arias and CISO-A Research Team, 2010 were used for data collection. The obtained results revealed that there is a positive relationship between problematic internet use and social anxiety. Males were found to have more social anxiety and problematic internet use. Further it is found that there is no significant different between Problematic Internet use on the basis of locality, but were found significant different social anxiety.

Keywords: Problematic Internet Use, Social Anxiety, Gender and Locality

4

Development and Validation of High Performance Liquid Chromatography for the Determination of Domperidone and Esomeprazole in Their Pharmaceutical Formulation

Ahmed S. A. Ali1,*, Omnia I. M. Ali2, Abdel Fattah B. Farag2, Maged F. Abdel Khalek3

1. Hefny Pharma Group, Cairo, Egypt

2. Chemistry Department, Faculty of Science, Helwan University, Ain Helwan, Cairo, 11795, Egypt

3. Pharco Pharmaceutical Company, Alexandria, Egypt

ABSTRACT

A new precise, accurate, robust and stability indicating high-performance liquid chromatographic method has been developed and validated for the simultaneous estimation of domperidone (DOM) and esomeprazole (ESO) in their pharmaceutical formulation. The proposed method carried out on Waters Symmetry C18 column (250 mm x 4.6 mm, 5.0 µm particle size) using an isocratic elution technique at a column temperature of 30 ?C. The mobile phase was a mixture of 0.01M sodium acetate buffer: methanol (45:55 v/v) and it was adjusted to pH 4.5 using glacial acetic acid with a flow rate of 1 mL/min and an injection volume of 20 µL. The retention times were 3.7 and 5.0 min with UV detection at 290 nm for DOM and ESO, respectively. The proposed method was linear over the concentration ranges of 0.04-60.0 and 0.08-120.0 µg/mL for DOM and ESO, respectively. Limit of detection and limit of quantitation values were 0.48 and 1.44 µg/mL for DOM and 0.47 and 1.43 µg/mL for ESO, respectively. The method also exhibited good levels of recovery from 100.23% to 101.59% for DOM and from 99.74% to 101.57% for ESO. From the validation study, it was found that the method was specific, rapid, accurate and reproducible. The high percentage of recovery and low relative standard deviation confirm the suitability of the method for routine pharmaceutical analysis of both drugs separately or in their combined dosage form.

Keywords: HPLC; Esomeprazole; Domperidone; Validation; Formulation.

5

Antioxidant, Free Radical Scavenging and In Vitro Cytotoxic studies of Different Solvent Extracts from the Stem Bark of Bauhinia Variegata Linn.

Trupesh Pethani1*, Mayuri Thumar2, Vishal Airao1, Ashvin Dudhrejiya2

1. Department of Pharmaceutical Sciences, Saurashtra University, Rajkot, Gujarat, India.

2. B.K. Mody Government Pharmacy College, Rajkot, Gujarat, India.

ABSTRACT

Bauhinia variegata (Family: Fabaceae) is well-known medicinal plant used from the ancient era to till date for their medicinal values. The powerful biological activities as exhibited by plant flavonoids posed the need of determining their contents in B. variegata stem bark. In view of its wide use and its chemical compositions, this study was aimed at examining and comparative analysis of the antioxidant and anticancer activities of the different extracts of stem bark. Antioxidant activity of extracts was expressed as percentage of DPPH, super oxide and nitric oxide free radicals inhibition and expressed asIC50 values (?g/mL). Methanolic extracts of B. variegata showed the highest amount of and ?avonoid contents and reducing capacity whereas, chloroform and dichloromethane extracts of B. variegata showed pronounced cytotoxic effect against HCT-116, A549 and ethyl acetate extracts showed pronounced cytotoxic effect against Ovcer-5 human cancer cell lines. The order of antioxidant activity in B. variegata extracts displayed from higher to lower level as methanol, hydro-alcoholic, ethyl acetate, chloroform, dichloromethane and n-hexane extracts of stem bark of B. variegata. Commercial standards were taken as control showed highest antioxidant power in the present study. Chemical components of B. variegata have good antioxidant capacities and this species could be used as a potential source of new natural antioxidants.

Keywords: Antioxidant; Bauhinia variegata; Cytotoxicity; Stem bark extract; Total flavonoids.