AJPHR

American Journal Of Pharmacy And Health Research

ISSN NO.: 2321-3647
DOI: 10.21276/ajphr
October 2018 Issue 10
1

Preformulation Study of Dolasetron: An Insight for Formulation and Development of Nasal Formulation

Mansi Rathod, Dharmik Mehta, Pragna Shelat, Punit Parejiya

Department of Pharmaceutics, K. B. Institute of Pharmaceutical Education and Research, Kadi Sarva Vishwavidyalaya, Gandhinagar, Gujarat, India

ABSTRACT

The objective of preformulation study is to develop the elegant, stable, effective and safe dosage form by establishing kinetic profile, compatibility with other formulation excipients and to establish physico-chemical parameter of new drug substance. This could provide important information for formulation design or support the need for molecular modification. So, in the present study preformulation studies were performed on Dolasetron (DS) to assess its suitability for nasal formulation. DS is a specific and selective serotonin subtypes 3 (5-HT) receptor antagonists, used to treat chemotherapy induced nausea and vomiting. The authenticity of DS was established by DSC and FITR spectra. An UV spectrophotometric method and HPLC method were employed for determination of DS in bulk and blood plasma respectively. Saturation solubility, micromeritical properties, melting point, pH, hygroscopicity and stability profile were studied. The UV method was linear in the range of 5-50 ?g/ml. The low % CV values of intra-day and inter-day variations revealed that the proposed method is robust. The retention time of DS in HPLC method was found to be 2.8 min. The method was proven robust by obtaining very high regression coefficient value (0.999). The results of the physicochemical study of drug revealed suitability of DS for nasal route. Moreover, the drug was found stable in both solid as well as liquid state at different conditions.

Keywords: Preformulation, dolasetron, nasal formulation, bioavailability, stability

2

Chemical Screening, Antioxidant Potential and Antiangiogenic Effect of Microporus Xanthopus (fr.) Kuntze, Ganoderma Orbiforme (fr.) Ryvarden and Polyporus Fasciculatus (pat) lloyd, Medicinal Mushrooms from Gabon

Orango-Bourdette Juliette-Ornely1,2, Eyi-Ndong Hugues Calixte3, Ndong-Atome Guy Roger1,2, Ngoua Meye Misso Rick-Léonid1,2, Sima-Obiang Cédric1,2, Ondo Joseph-Privat1,2, Obame-Engonga Louis-Clément1,2*

1.    Laboratory of Research in Biochemistry (LAREBIO), University of Sciences and Technology of Masuku, Franceville, Gabon.

2.   Laboratory of Natural Substances and Organometallic Synthesis (LASNSOM), University of Sciences and Technology of Masuku, Franceville, Gabon.

3. Institute of technology Research (IRT), Cenarest-Libreville, Gabon

ABSTRACT

The objective of the present study was investigation of the chemical screening, antioxidant potential and antiangiogenic effect of Microporus xanthopus, Ganoderma orbiforme and Polyporus fasciculatus, medicinal mushrooms from Gabon. Theantioxidant activities of water, water-ethanol and ethanol extracts of three fungi were tested by measuring the trapping power of DPPH radical. The anti-angiogenic activity was evaluated for some water extracts by chicken chorioallantoic membrane method (CAM). The results obtained for DPPH trapping test showed that the various extracts tested had antioxidant activities going from low to very high. The greatest activities were found in Microporus xanthopus with IAAs of 3.88 and 4.13, respectively, for water and water-ethanol extracts. Polyporus fasciculatus fungi exhibited strong antioxidant activities. The moderate activity was found in the extract of Ganoderma orbiforme. The water extracts tested for their anti-angiogenic activity acted by decreasing the density and/or number of blood vessels of the CAM with inhibition percentages of 66.67%, 55.45% and 37%, for Ganoderma orbiforme, Microporus xanthopus and Polyporus fasciculatus, respectively. Thus, angiogenesis can be neutralized by the antioxidant molecules contained in the fungi, but these molecules are not the only ones to possess these potentialities. Harvested mushrooms could be potential agents for the fight against cancer.

Keywords: Cancer, antioxidant and anti-angiogenic activities, phenolic compounds, CAM, mushrooms.

3

Formulation and Comparative Standardization of Polyherbal Swadisht Virechan Churna

H. A. Navthale*, M. B. Bhaltadak, R. R. Thenge, S.A. Shinde, V. S. Adhao

Dr. Rajendra Gode College of Pharmacy, Malkapur-443101, Maharashtra, India

ABSTRACT

Standardization of herbal formulation is essential in order to check quality, purity, safety and efficacy of drug. “Swadisht Virechan” churna play an important role in constipation and detoxification due to safety and efficacy in it. Hence churna has been formulated by standard procedure and evaluated by organoleptic study, physical characteristics and physicochemical screening. One marketed formulation and one lab scale formulation were subjected to comparative standardization. The churna was standardized by determination of organoleptic characters, pH, loss on drying, ash value, extractive value, physical characteristics such as bulk density; tap density, angle of repose to determine flowability, determination of particle size, microbial content. The result of various parameters obtained from study showed that marketed formulation and lab scale formulation have comparable physical values. The flowability of formulation was found to be poor in both formulations. These studies showed that there is no uniformity in preparation of formulation which is may be due to varied geographical locations where there plants grow. The present paper reports the investigation and standardization of swadisht virechan churna an Ayurvedic formulation. The physical parameter evaluated confirms the standard of the formulated churna.

Keywords: “Swadisht Virechan” churna, Ayurvedic formulation, Standardization.