Since ancient times, plants, especially herbs have been used for their medicinal properties by us humans. This practice of using plants for their medicinal property gave rise to the science of Ayurveda. These herbs not only have medicinal value but are also used as dietary supplements. Today such supplements are widely accepted and used by people all over the world. There are various types of dietary supplements available in the market today. However, this formulation is innovative and unique. It is a dietary supplement which is suitable for all ages (except infants) and especially palatable for children. It can be taken anytime and is also rich in vitamins and minerals. It is a herbal extrudate made up of different herbs. The herbs were selected on the basis of availability which includes spinach, turmeric, ginger, liquorice etc. The extrudates contain equal amounts of herbs. A moist mixture is prepared using suitable vehicle (water). The moist mixture upon passing through an extruder forms extrudates which are then dried and packed. The extrudates were studied for their physico-chemical properties. And hence, the results were interpreted accordingly which were found to be significant.

Keyword: Vitamins, liquorice, turmeric, ginger

Ciprofloxacin (CPFX) and Tetracycline (TC) are two of the most widely used antibiotics in human and veterinary medicines. Their existence in the environment has experienced much attention because of the potential adverse effects on humans and ecosystem functions. In this paper, the interaction mechanism between the two antibiotics and Human holo transferrin by spectroscopic and molecular docking methods. The distance r, between the antibiotics and the protein was obtained according to FRET which pointed at a successful formation of a drug – protein complex. Molecular docking study provided possible binding sites of antibiotics within the proteins. The obtained data can be convenient for determining usage drug doses in drug delivery.

Keywords: Human holo transferrin, ciprofloxacin, Tetracycline, FRET, Molecular Docking.

ABSTRACT

A simple and precise, reliable, rapid, reproducible reverse phase HPLC method has been developed and validated for simultaneous estimation of Probenecid & Cefadroxil chromatography was carried out on zorbaxcC18 (4.6*150mm, 5um) column using mixture of ACN: WATER (55:45v/v) as the mobile phase at flow rate of 1.0ml for min the detection wavelength carried out at255nm the retention time of probenecid & cefadroxil was 2.061&2.462respectively. The method produce linear response in the concentration range of 10-50µg/ml of probencid&5-25µg/ml of cefadroxil the method precision for the determination of assay was below 2.0%RSD. The proposed method can be useful in quality control of bulk& pharmaceutical dosage form

Keywords:  Probenecid, Cefadroxi, Rp-hplc validation