ABSTRACTThe aim of the present work was to develop and validate a simple and efficient method for the analysis of Aprepitant in pharmaceutical dosage forms by reverse phase high-pressure liquid chromatography. A stainless steel column 75 mm long, 4.6 mm internal diameter filled with octasilyl silica chemically bonded with synthetic hybrid silica gel particles of 3.5 mm diameter was used for elution. The retention time of Aprepitant was 4.05 min. The method showed a good linearity in the concentration range of 0.02478 – 0.07434 mg/mL with a correlation coefficient of 0.9999. The validation characteristics included specificity, linearity, limit of detection, limit of quantification, precision, robustness and stability. Validation acceptance criteria were met in all cases. The method could be successfully used for the analysis of Aprepitant in pharmaceutical dosage forms. Keywords: Aprepitant, Accuracy, Precision, Linearity, Mobile Phase and Validation