A new, rapid sensitive, simple and cost effective UV method was developed for the estimation of balofloxacin in bulk as well as in pharmaceutical formulations. The absorbance of balofloxacin was measured in 0.1N sodium hydroxide at new wave length (λmax 290). The linearity range was found to be 1-9 μg/ml.The method was tested and validated for various parameters as per ICH specification. The result demonstrates that the developed procedure is accurate, precise and reproducible. Proposed method is applicable for the estimation of balofloxacin in different dosage forms and results are in good agreement with label claim. Key words: Balofloxacin, 0.1N Sodium hydroxide, UV method.