The root bark of Plumbago zeylanica Linn has potent analgesic and anti-inflammatory activity which has been used in many formulations of Indian system of medicine in treating Cancer. Since less information is available on the effects of hydro alcoholic extract of Plumbago zeylanica on inflammation and nociception, the present study was designed. To study the analgesic and anti-inflammatory activity of the hydro alcoholic extract of root bark of Plumbago zeylanica Linn. Soxhlet extraction of root bark powder done using 85% Methanol and Water 15% to obtain test drug. Analgesic effect was studied using hot plate and tail immersion analgesia in rat. Anti-inflammatory activity was investigated through in vitro Human Red Blood Cell Membrane protective activity and, Carrageenan induced rat paw edema and Complete Freund’s Adjuvant induced chronic inflammatory model in rat. Analgesic activity of the extract was studied by hot plate and tail immersion method revealed significant activity at 350 mg/kg b.wt dosage on comparing with weak analgesic standard drug Paracetamol. Studies of extract on both acute and chronic model of inflammation induced by Carrageenan and Complete Freund’s Adjuvant in rat showed moderate anti -inflammatory action at primary phase of inflammation at the dosage of 250 mg/kg b.wt comparable with standard Indomethacin.