New series of (E)-methyl-2((E)-2-((Z)-(3-methyl-2,6-diarylpiperidin-4-ylidene)hydrazono)-4-oxothiazolidin-5-ylidene)acetate have been synthesized from 2,6-diaryl-3-methyl-piperidin-4-one as a starting material by microwave method.  Excellent yield was obtained in shorter reaction time as these reactions were carried out under microwave irradiation, it reduces the cost and time period of reaction.  The synthesized compounds exhibited moderate to strong antibacterial activity and antifungal activity against some selected bacteria and fungi. The structures of all the synthesized compounds were confirmed by chemical and spectral analysis such as IR, 1H NMR, 13C NMR and mass spectroscopy.