Ultraviolet-B radiation affects the surface layer of the skin mainly Keratinocytes which leads to harmful biological disorder. This study explored the effect of Methanolic extracts of Fish Mucus (FME) from parrot fish Scarus ghobban against UV-B radiation treated Cultured Human Keratinocytes (HaCaT). HaCaT cells treated with FME for 24hrs followed by UV-B irradiation for 15mJ/cm2. After the irradiation, the HaCaT Cells were assayed for IL-2 cytokine. UV-B radiation induces an increase in cytokines, when ti is incorporated with the FME the concentration of cytokines reduced to normal. This study reveals that FME may be a photoprotective agent.