Nonsteroidal anti-inflammatory (NSAIDs) drugs are generally used as a part of drug therapy in inflammation. However, these drugs have severe side-effects. Therefore, naturally originated compounds with very little side-effects are required to substitute chemical therapeutics and have better safety profile. Solanum sisymbriifolium (Family: Solanaceae) has many therapeutic uses mentioned in Ayurveda. Moreover, the plant has diverse pharmacological activity such as antihypertensive, diuretic, analgesic, contraceptive, antisyphilitic, hepatoprotective. Therefore, the present study was designed to investigate the preliminary phytochemical screening and anti-inflammatory activity of methanolic extract of roots of Solanum sisymbriifolium (MERS). Carrageenan-induced rat paw edema model was used for assessing the anti-inflammatory activity. The phytochemical analysis of MERS confirmed the presence of alkaloids, carbohydrates, flavanoids, terpenoids, tannins and saponins. Furthermore, MERS at doses of 50,100, 200 and 300 mg/kg showed anti-inflammatory activity in dose-dependent manner.