Triphala-guggula vati a polyherbal Ayurvedic formulation is recommended in the management of pain in renal stone, it has dual action of aiding digestion and lowering body weight. It is effective in lowering cholesterol and reducing fat. It is also use in treatment of anal fistula, fissures and severe constipation. There has been an increase in demand for the Phyto-pharmaceutical products of Ayurveda so a pharmaceutical preparation in the form of Triphala-guggula vati was tried to standardize in terms of Fingerprinting and Stability studies, which is economical in terms of time and machinery usage. Literature survey indicates that no Fingerprinting and stability studies were carried out; hence present study was undertaken for the same. UV and HPTLC studies were carried out for finger printing of all important phytoconstituents of Triphala-guggula vati and its raw ingredients. HPTLC study were carried out on Aqueous and Alcoholic extracts using Camag HPTLC system equipped with Linomat V applicator, Camag TLC scanner 3 and CATS 4 software for interpretation of data. The laboratory batch and two Marketed preparations of Triphala-guggulu vati were subjected for development of rapid, reproducible, valid Fingerprinting method and stability testing method at standard recommended accelerated condition.