Most of the industrial effluent contains various chemical compounds such as chromium, which is pulmonary carcinogen and health hazardous to living system. Generally, the industrial effluent is alkaline, hence, attempt was made to isolate the bacteria from alkaline environment, having ability to survive and remediate the chromium. In the present study, twelve sample of water, matt and sediment were collected from alkaline Lonar Lake and bacterial strain were isolated in nutrient broth (pH 10) containing 100μg/mL of chromium. The bacterial isolate was characterized morphologically and biochemically and identified as Lysinibacillus mangiferihumi by 16S rRNA gene sequencing. The chromium remediation ability of the strain was estimated by the Spectrophotometric method of Di-phenyl carbazide and it showed that 89% of chromium reduction in 96 h of incubation. Thus, it indicated that the isolated Lysinibacillus mangiferihumi bacillus could remediate and detoxify hexavalent Chromium (VI) to trivalent Chromium (III) in alkaline environment.