The ivermectin is mostly used an anti-parasitic drug for treatment of Argulosis and other ectoparasite diseases. The present study was aimed to investigate the effect of acute exposure (96 h) of different concentration of ivermectin in the water on metabolism and blood glucose level of rohu (Labeo rohita) fingerlings. The graded level of ivermectin such as 2.5, 5.0, 7.5, 10.0, 12.5, 15.0 and 17.5 μg/L in water were selected and a test was conducted in triplicate for each concentration with 10 fishes in each tub of 100 L capacity. At the end of the experimental period of 96 hrs, activity of alkaline phosphatase (ALP) and blood glucose level were significantly decreased (P < 0.05) with respect to concentration of ivermectin, while the activities of  lactate dehydrogenase (LDH) and transaminase enzymes (alanine amino transferase and aspartate amino transferase) were significantly increased  (P < 0.05) in exposed groups compared to the control. A negative correlation was recorded between the level of ivermectin concentration in water and blood glucose level. The gross observations noticed were sluggishness, inactivity, dark body color, disorientation and imbalance in swimming.