The Mimosa pudica Linn. is a plant having multi activity and has been using as a single herb or as in polyherbal preparation from the prehistoric time. The plant is claimed to be Anthelminthic, antihyperglycemic, anti-inflammatory, antipyretic, antispasmodic, antitussive, antiviral, calmative, contraceptive, depilatory, diuretic ,emetic, expectorant , poison, sedative and tranquilizing by different traditional practitioner, different community and in different literature. While in Ayurveda it was mentioned that the decoction of the whole plant was used to wash the vaginal infections. From different literature, it is come to know that in Bangladesh and in Sudan it is used in UTI and Oral infections. A clear justification arises that the plant may have antimicrobial activity.  Finally plant were tested against six microbial strain out of which two were gram positive bacteria, two were gram negative bacteria and two were fungal strain. Staphylococcus aureus  MTCC 3160, Straphylococcus saprophyticus MTCC 96 , Klebsiella pneumonia MTCC 4032, Escherichia coli MTCC 1303, Aspergillus niger MTCC 281 & Candida albicans MTCC 1637 obtained from department of microbiology, down town hospital, Guwahati were the choice of test organisms. The inhibition of zone were tested using the disc diffusion method. Ciprofloxacin and Fluconazole were used as positive control for the bacterial and fungal strain respectively whereas DMSO was used as negative control. The activity indexes were determined.