Our aim in the present study was to evaluate the anti-obesity activity of Centella asiatica in high fat diet (HFD), Triton-X and Progesterone induced obesity in rats and mice. Ethanolic extract of Centella asiatica (EECA) was prepared and the extract is tested with different doses (100, 200 and 400mg/kg) and the efficiency of EECA is comparable to that of standard anti-obesity drug Orlistat (20mg/kg). Although food consumption was moderately increased in high fat diet fed rats, EECA administration significantly reduces weight gain in them. Serum total cholesterol (TC), triglycerides (TG), low density lipoproteins (LDL) and very low density lipoproteins (VLDL) levels were significantly (P< 0.05) lowered, while high density lipoproteins (HDL) increased in EECA administered rats. Based on our results, we find that the EECA has potential anti-obesity activity.