Peptic ulcer is a one of the major ailment effecting 10% of the population. Drugs are found by screening compounds against an animal model of human disease. Main aim of the present study is to evaluate anti-ulcer activity of cobalt and nickel derivatives of 3-methoxysalicylaldehyde-2-aminobenzoylhydrazone by pylorus ligation method in albino rats. Anti-ulcer activity was compared with standard drug diclofenac sodium. In pyloric ligation induced ulcer model, the studied parameters were gastric volume, pH, total acidity, free acidity, ulcer index and percentage of ulcer protection. In treated groups of nickel and cobalt derivatives total acidity and ulcer index were significantly decreased, gastric pH was increased and there is no significant change in volume of gastric acid secretion, free acidity as compared to control group. Percentage protection increased dose dependently in animal treated with both the derivatives. Group treated with Nickel derivative showed good results as compared to cobalt derivative treated group. These results indicate that both nickel and cobalt quinazolinone derivatives have better potential against ulcer.