The present study was aimed to investigate the phytochemical and anthelmintic effect of aqueous and acetone extracts of Andrographis paniculata leaves on Indian earth-worm Pheretima posthuma. The aqueous and acetone extracts were prepared and various phytoconstituent tests were conducted to find the compounds present in the leaves of A. paniculata. Then in vitro activity is determined. Three concentrations (10, 50, 100 mg/ml) of each extract were studied in activity which involved the determination of time of paralysis and time of death of the worms. The leaves extracts of A. paniculata which contains the various phytoconstituents such as tannins, flavonoids, alkaloids etc. Both the extracts exhibited significant anti-helmintic activity in a dose dependent manner. In present investigations confirms that the leaves extracts of A. paniculata contains various phytoconstituents and this leaves which contains the anthelmintic activity against the Pheretima posthuma.