Formoterol fumarate is a selective long acting β2 adrenergic receptor agonist indicated for the treatment of chronic obstructive pulmonary disease (COPD). Various analytical methods used for the estimation of Formoterol fumarate alone and combination with other drugs have been reviewed in this paper. These are  include Ultraviolet spectrometry, High performance thin layer chromatography, High performance liquid chromatography, Ion pair to determine the amount of Formoterol fumarate in bulk drugs and pharmaceutical formulations as well as in Biological Fluids. Stability indicating and impurity profiling methods for Formoterol fumarate are also described. These analytical methods can be used for qualitative and quantitative estimation of Formoterol fumarate and / or its related impurities or degradants in bulk and formulation.