ABSTRACTFor various health related problem medicinal plant oils are widely used in traditional Indian society. In present study Antimicrobial activity of different crude oils compare with standard medicinal oils. Crude oils are extracted from different parts of plants such as leaves of Eucalyptus globulus (Eucalyptus oil), Flower buds of Eugenia caryophyllus (Clove oil), Leaves of Cymbopogen citritis (Lemon grass oil), seeds of Recinus communis (Castor oil), and seed of Sesamum indicum (Sesam oil) by using Steam distillation process. The antimicrobial activity of these crude oils with standard medicinal oil was studied by disc diffusion method, against one gram +ve Bacterial species Bacillus subtilis,two gram -ve Klebsiella pneumonia & Pseudomonas aeruginosa and one fungal strain Aspergillus niger. Comparing zone of Inhibition of crude oils with standard oils and also perform the phytochemical test for crude & standard oils.  Keywords: Antimicrobial activity, Clevenger apparatus, Medicinal plant oil, phytochemical test