ABSTRACTThe objective of the present study was investigation of the chemical screening, antioxidant potential and antiangiogenic effect of Microporus xanthopus, Ganoderma orbiforme and Polyporus fasciculatus, medicinal mushrooms from Gabon. Theantioxidant activities of water, water-ethanol and ethanol extracts of three fungi were tested by measuring the trapping power of DPPH radical. The anti-angiogenic activity was evaluated for some water extracts by chicken chorioallantoic membrane method (CAM). The results obtained for DPPH trapping test showed that the various extracts tested had antioxidant activities going from low to very high. The greatest activities were found in Microporus xanthopus with IAAs of 3.88 and 4.13, respectively, for water and water-ethanol extracts. Polyporus fasciculatus fungi exhibited strong antioxidant activities. The moderate activity was found in the extract of Ganoderma orbiforme. The water extracts tested for their anti-angiogenic activity acted by decreasing the density and/or number of blood vessels of the CAM with inhibition percentages of 66.67%, 55.45% and 37%, for Ganoderma orbiforme, Microporus xanthopus and Polyporus fasciculatus, respectively. Thus, angiogenesis can be neutralized by the antioxidant molecules contained in the fungi, but these molecules are not the only ones to possess these potentialities. Harvested mushrooms could be potential agents for the fight against cancer. Keywords: Cancer, antioxidant and anti-angiogenic activities, phenolic compounds, CAM, mushrooms.