ABSTRACTThe present study was carried out to evaluate preliminary phytochemicals and wound healing activity of hibiscus cannabinus seed of ethanolic aqueous extract. In this study we observed that percentage yield of ethanolic extract was 8.5% w/w and the extract was subjected to TLC analysis and also carried out the estimation of total phenolic and flavonolic content. In this study we observed that no toxic symptoms like behavioral changes, locomotion, convulsions etc. were observed. Also, we calculated the  values. For the conducting wound healing activity we utilized excision and incision wound models. Keywords: Hisbiscus cannabinus L. seed, locomotion, TLC analysis,  values, incision and excision wound models.