ABSTRACTA series of N-{[2-(substituted methylidene)hydrazinyl](methylsulfanyl)methyl}-2-substituted-1,3-thiazolidine-4-carboxamide derivative were synthesized. The synthesized compounds were characterized by IR, NMR, Mass spectral data. The synthesized compounds were screened for their antifungal and antibacterial activity against pathogenic fungus and bacteria. Compounds TS-2, TS-4 and TS-9 exhibited good antibacterial and antifungal activity against the tested microorganisms, and the  rest of the molecules  shows good to moderate activity. Keywords: substitute thiazolidine, microwave assisted synthesis, minimum inhibitory concentration, antibacterial, antifungal.