ABSTRACTSanitizer are available in market, but contain chemical, that’s why they give side effects. So, attempt to prepare herbal hand sanitizer. Herbal hand sanitizer are used to show antimicrobial activity against, viruses, fungi, pathogens, and (bacteria - E.coli, pseudomonas aeuroginosa, staphylococcus aureus, and Bacillus subtilis). Herbal sanitizer prepared from herbal drugs. So it’s having no side effects as compared to standard. Using medicinal herb and plant with human has been known since the old civilization. Old drug industry depend on raw materials of medicinal herbs and plant and their extract, which always proved safe. Herbal formulation is always use as protective purpose by micro-organisms such kind of this formulation is helpful to protect from bacteria and virus and it’s should have capacity to kill micro-organism, bacteria, viruses, fungi etc1. Keywords: Sanitizer, Parsley, Microorganism, Herbal drug, Zone of inhibition, E. coli.