The synthesis of a series of 2-anilinophenylacetic acids, close analogue of diclofenac, is synthesized and tested for their anti-microbial activity by disk diffusion method. Each 2-anilinophenylacetic acid derivative will be screened for their anti-microbial activity. Syntheses have been carried out following simple methodology in excellent isolated yields. It has been found that compounds AB11, AB12, AB14, AB21, AB24, AB25, AB32, AB34, AB35, AB44, AB45 and AB54 showed the good activity against both of strain comparable to ampicillin and ciprofloxacin was taken as standard at the same concentration whereas all the other compound has showed mild to moderate anti-microbial activity as compared to standard drug. The structure and purity of the original compounds were confirmed by Melting Point, IR, TLC and elemental analysis. Each 2-anilinophenylacetic acid derivative will be screened for their anti-microbial activity. These preliminary results indicate that some of compounds are exhibiting good activity. -------------------------------------------------------------------------------------------------------------------------------------