A Liquid Chromatography method for the Simultaneous Determination of Safranal and Piperine in Some Marketed Proprietary Chyawanprash Products

A rapid and simple HPLC method has been developed for the simultaneous quantification of safranal and piperine in some marketed proprietary Chyawanprash products. Analysis was performed using C18 column (250 x 4.6 mm) by isocratic elution with acetonitrile: water (77:23) and detection at 308 nm using Ultra Violet (UV) detector. The calibration plot was linear over the range studied (safranal: 0.5-10 ppm; piperine: 2.5-50 ppm) with a correlation of 0.999 for safranal and 0.999 for piperine. The method was also validated for the linearity, range, precision, recovery and detection limits. Thus, the method is suitable for routine analysis of safranal and piperine in marketed proprietary Chyawanprash products.