Preparation of Suitable Nanofibre by Blending of Natural and Synthetic Polymer for Tissue Engineering Applications

Tissue engineering field is an interdisciplinary field that has endeavoured to utilize a variety of processing methods with synthetic and natural polymers for various applications like scaffold preparation, drug delivery, wound healing and nanoparticle preparation. Blending of Polymers is one of the most effective method for providing new, desirable biocomposites for tissue engineering applications. Polymer exhibits a variety of physical and chemical properties from the constituent polymer. In this project preparation of chitosan and polyethylene oxide nanofibrewas performed by the process of electrospinning method. Chitosan is a natural polymer obtained from shell of shellfish and it has novel properties like biocompatibility, biodegradability, and antibacterial. Polyethylene oxide is a synthetic polymer which is also reported to be non toxic and highly degradable polymer. Blending ratio of these two polymers was varied to obtain uniform nanofibres. The blended polymer had been characterized by SEM, UTM and FTIR studies and their toxicity was studied by MTT assay and staining method.