Development and Validation of a UV Spectrophotometric Method for Analysis of Dexmedetomidine HCL

A simple, accurate, rapid and reproducible UV-Spectroscopy method have been developed and validated for the estimation of Dexmedetomidine HCl in pure and dosage form. The absorbance of drug measured at 214nm wavelength in 0.9% NaCl solution. The range of linearity was found to be 2-10 µg/mL with the linearity equation of Y=0.0499x + 0.0517 and correlation coefficient is 0.9988. Developed method was validated according to the ICH Q2(R1) guidelines. The % RSD values for interday and intraday were found to be less than 2%. And % Recovery were in between 99.88-100.12%. LOD and LOQ were found to be 0.060 and 0.183 respectively. The result concluded that the developed method is accurate, precise and reproducible. Key words: Dexmedetomidine HCl, 0.9% NaCl, UV Spectrophotometric method.