Analytical Method Development for simultaneous estimation of Calcipotriene and Dipropionate in Pharmaceutical dosage form.

A new method was established simultaneous estimation of calcipotriene and dipropionate by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of calcipotriene and dipropionate by using coloumn xterr C-18(4.6*250mm) 5µm, flow rate was 1.0ml/min mobile phase ratio Phosphate buffer (0.05M) pH 3.6: ACN (40:60%v/v) (pH was adjusted with ortho phosphoric acid), detection wave length 260nm.the instrument used was water HPLC auto sampler and PDA or detector. The analytical method was validated to ICH guidelines. The linearity range Dipropionate   and Calcipotriene were found to be from 100-500 µg/ml of Dipropionate and 1-5µg/ml of Calcipotriene. Linear regression coefficient was not more than 0.999.