Enhanced Tannase Production Using Aspergillus Flavus by Fed-Batch Fermentation with Redgram Husk

Tannin acyl hydrolase also known as tannase (EC 3.1.1.20) is a hydrolytic enzyme that catalyses the hydrolysis of (hydrolysable) tannins such as tannic acid, resulting glucose and gallic acid. The enzyme has wide applications in food, beverage, brewing and chemical industries. It is mainly used for the preparation of Gallic acid, instant tea, wine, coffee flavored soft drinks, clarification of beer and fruit juices. Enhancement of tannase production is required to meet its current demand. The aim of the present study is to enhance the production of tannase enzyme using Aspergillus flavus (MTCC 3783) using Redgram Husk as substrate in a 3L fermenter by batch and fed-batch fermentation. By applying a fed-batch strategy, production of tannase could be almost doubled as compared to Batch fermentation using the substrate Redgram Husk with the yield of 160.14 U/ml and 88.46 U/ml respectively. The maximum production of tannase using Redgram Husk showed the suitability of this culture process for industrial-scale development.