Probiotic profiling of Lactobacillus spp. isolated from the intestine of Sperata seenghala and Labeo bata

A very common probiotic bacterium is Lactobacillus. In the current study for the first time the isolation has been done from Sperata seenghala and Labeo bata. The objective of the current study was to isolate and identify Lactobacillus bacteria from the intestines of these two fishes. The total bacterial counts were 2.1x106 and 1.8x105 CFU/g. Isolation was done by dissection, homogenization and filtration. The isolates were identified as Lactobacillus by gram staining, microscopic observation and biochemical tests. The isolates showed growth within pH 3 to 8 with maximum growth at pH 7. They stood firm at bile salt concentrations of 0.0, 0.1, 0.2 and 0.3%. Antibiotic sensitivity test was done by disc diffusion method. The Lactobacillus isolated from S. seenghala was resistant to Ampicillin, Ceftazidime, Cefuroxime, Co-trimoxazole and Nalidixic Acid. Isolate from L. bata was resistant to Ampicillin, Azithromycin, Ceftazidime, and Cloxacillin. Both these isolates can be denoted as potential probiotics and they will serve as very useful tools for future research.