Ashish N. Phuge

Three simple, accurate and precise UV- Spectrophotometric methods has been developed and validated for quantitative determination of Gatifloxacin (GTX) and Ketorolac Tromethamine (KTA) in bulk and marketed formulation. First method is based on simultaneous equations and the wavelengths selected for analysis were 292 nm (λmax of Gatifloxacin) and 319 nm (λmax of Ketorolac tromethamine). Second method is based on the ratio of absorbances at two selected wavelengths, one at isoabsorptive point and the other being λ max of one of two components. Third method is based on area under curve and the wavelengths ranges selected for analysis were 290-294 nm for Gatifloxacin and 317-321 nm for Ketorolac tromethamine. Linearity was obtained in the concentration range of 2-12 μg / ml for both drugs. The results of analysis have been validated statistically and by recovery studies. The utility of the developed methods has been demonstrated by analysis of commercial formulation containing these drugs.