Konda Ravi Kumar

ABSTRACTCancer is a group of diseases involving uncontrolled and abnormal growth of cells with potential to invade (or) spread to other parts of body. The aim of present study is to evaluate the invitro anti cancer screening of ethanolic extract of whole plant of Eclipta alba against HL-60 cell lines by SRB (sulphorhodamine) assay, besides this property it has many other therapeutic uses. The plant with methanol is kept in closed vessel for seven days and the obtained extract is subjected to preliminary phytochemical analysis and in vitro anti cancer screening, these test reveals the presence of secondary metabolites and LD50 concentration of extract was >80 µg/ml respectively i.e) the extract can inhibit the growth of cancer cells rather than killing them. For killing the cancerous cells very high concentration of extract is required. Key words: Eclipta alba, Anti-cancer activity, SRB assay, HL-60 cell lines.

An isocratic reversed-phase liquid chromatograpic assay method was developed for the quantitative determination of Atorvastatin calcium (ATOR) and Clopidogrel bisulphate (CLOP) in combined dosage form. A Zodiac C18, 250x4.6mm, 5µm column and OPA Buffer: ACN (70: 30,v/v) as mobile phase. The flow rate was 1mL/min and effluents were monitored at 241 nm. The retention times of Atorvastatin and Clopidogrel were 5.8 min and 3.5 min respectively. The correlation coefficient was found to be 0.99947 (for ATOR) and 0.99944 (for CLOP). The proposed method was validated with respect to linearity, accuracy, precision, and robustness. The method was successfully applied to the estimation of Atorvastatin and Clopidogrel in combined capsule dosage form.