Development and Validation of HPTLC Method for Simultaneous Estimation of Atorvastatin Calcium and Aspirin In Bulk and Dosage Form

A simple, accurate, and precise HPTLC method has been developed and validated for the simultaneous estimation of Atorvastatin Calcium (ATO) and Aspirin (ASP) from bulk drug and Dosage form. The method employed TLC aluminum plates precoated with silica gel 60 GF 254 as the stationary phase. The solvent system comprised Toluene: Ethyl Acetate: Methanol: Acetic acid [7:2:1.5:0.1v/v/v/v]. This system was found to give good result for both the drugs (Rf value: of ASP 0.43cm and ATO 0.53cm). Spectrodensitometric scanning-integration was performed at a wavelength of 235nm.The calibration curve was found to be linear within the concentration range of 20ng/spot to 100ng/spot for ATO and 30 to 150ng/spot for ASP. The regression data for calibration curve shows good linear relationship with r2 = 0.9989 and 0.9990 for ATO and ASP respectively. The method was validated in accordance with the requirements of ICH guidelines. The method was successfully applied for determination of drug in bulk and Dosage form. Thus, the proposed method can be used successfully for routine analysis of ATO and ASP from bulk and dosage form.