HPTLC

The objective of this study was to establish a reasonably simple and reliable method to estimate Poly (hexamethylene biguanide) hydrochloride [PHMB] in marketed formulation. New methods like UV & HPTLC were developed for estimation of Poly (hexamethylene biguanide) hydrochloride with all the validation parameters within range. Both the methods were developed and validated as per regulatory guidelines. UV method was developed in Water as a solvent. Linearity was found to be 2-12μg/ml for Poly (hexamethylene biguanide) hydrochloride. The method was found to be accurate, precise according to acceptance criteria. The proposed UV method can be applicable for the estimation of the drug in marketed formulation. HPTLC method was developed using mobile phase Methanol:O-phosphoric acid (10: 0.5 %v/v). In HPTLC method linearity was found 2000-12000ng/spot for Poly (hexamethylene biguanide) hydrochloride. HPTLC method was found to be simple, accurate and precise. The developed HPTLC method can be applicable for the estimation of the drug in marketed formulation. Developed methods can be applied in routine analysis for the estimation of Poly (hexamethylene biguanide) hydrochloride in marketed formulation.

To find out the secondary metabolites present in the ethanolic leaf extract of Ipomoea obscura (L.) by means of High Performance Thin Layer Chromatography (HPTLC). Ethanolic extract of the leaves were developed in the mobile phase of formic acid, water, n-hexane-ethyl acetate using standard procedures and scanned under UV at 366nm, 254nm and under visible light. The HPTLC fingerprinting of the ethanolic leaf extract of Ipomoea obscura (L.) showed the presence of 7 Flavonoids, 5 Alkaloids, 5 Terpenoids. From this analysis, it has showed that flavonoids are rich in Ipomoea obscura (L.). The intensive study on the out coming active constituents of Ipomoea obscura (L.) will lead to the discovery of a novel botanical drug.

‘Migraine’ comes from the Greek word, hemicranias, which means pain affecting one side of the head (classical Migraine) may affect the entire head. In Ayurveda Ardhavabhedaka there is severe headache in half portion of the head either left or right. So we can correlate Ardhavabhedaka with Migraine. Migraine (Ardhavabhedaka) is one of the most disabling of neurological disorders. The World Health Organization (WHO) has identified migraine among the world’s top 20 leading causes of disability. A clinical study was conducted on this problem with Brihat Jeevakadya Taila (BJT). It was inferred from the results that it was promising result effect in the treatment of Migraine. Till date there is no data regarding evaluation of BJT. It was aimed to develop the pharmacognostical and phytochemical profile of BJT. The samples were subjected to organoleptic, physicochemical analysis and High Performance Thin Layer Chromatography (HPTLC) examination by optimizing the solvent systems. The pharmacognostical study of ingredients of BJT shows the presence of Prismatic crystal, Stellate trichome, Oil globule, Border Pitted vessels etc. Pharmaceutical analysis showed that the loss on drying value was 0.1493% w/w, Specific gravity 0.9197, Refractive index 1.4780, Iodine value 72.21 and High Performance Thin Layer Chromatography at 254 nm and 366 nm resulted into 8 & 7 spots respectively. These parameters of pharmacognosy and pharmaceutical analysis can become the baseline for future.

A simple, precise, rapid, selective, and economic high-performance thin layer chromatography (HPTLC) method has been established for simultaneous analysis of Citicoline Sodium and Methylcobalamin. HPTLC method was developed using on precoated silica gel F254 G60 plates as stationary phase, using methanol: acetonitrile: water: triethylamine (8.5:1.5:1:0.5 v/v/v) as mobile phase. The plates were scanned at approximately 254 nm for both Citicoline sodium and Methylcobalamin respectively. In HPTLC method both the drugs were resolved using proposed mobile phase and Rf value was found to be 0.39 for Citicoline sodium and Rf 0.61 for Methylcobalamin. The method was found to linear in the range 1000-6000 ng/band for citicoline sodium and methylcobalamin respectively. This HPTLC procedure is economic, sensitive, and less time consuming than other chromatographic procedures. It is important tool for analysis of combined dosage form. Proposed method can be successfully applied for the quantitative determination of Citicoline Sodium and Methylcobalamin in Bulk drug and Pharmaceutical dosage form.

A simple, accurate, and precise HPTLC method has been developed and validated for the simultaneous estimation of Atorvastatin Calcium (ATO) and Aspirin (ASP) from bulk drug and Dosage form. The method employed TLC aluminum plates precoated with silica gel 60 GF 254 as the stationary phase. The solvent system comprised Toluene: Ethyl Acetate: Methanol: Acetic acid [7:2:1.5:0.1v/v/v/v]. This system was found to give good result for both the drugs (Rf value: of ASP 0.43cm and ATO 0.53cm). Spectrodensitometric scanning-integration was performed at a wavelength of 235nm.The calibration curve was found to be linear within the concentration range of 20ng/spot to 100ng/spot for ATO and 30 to 150ng/spot for ASP. The regression data for calibration curve shows good linear relationship with r2 = 0.9989 and 0.9990 for ATO and ASP respectively. The method was validated in accordance with the requirements of ICH guidelines. The method was successfully applied for determination of drug in bulk and Dosage form. Thus, the proposed method can be used successfully for routine analysis of ATO and ASP from bulk and dosage form.