Fingerprinting profile.

To find out the secondary metabolites present in the ethanolic leaf extract of Ipomoea obscura (L.) by means of High Performance Thin Layer Chromatography (HPTLC). Ethanolic extract of the leaves were developed in the mobile phase of formic acid, water, n-hexane-ethyl acetate using standard procedures and scanned under UV at 366nm, 254nm and under visible light. The HPTLC fingerprinting of the ethanolic leaf extract of Ipomoea obscura (L.) showed the presence of 7 Flavonoids, 5 Alkaloids, 5 Terpenoids. From this analysis, it has showed that flavonoids are rich in Ipomoea obscura (L.). The intensive study on the out coming active constituents of Ipomoea obscura (L.) will lead to the discovery of a novel botanical drug.