UV

ABSTRACTThe main objective of this study was to develop and validate an UV-spectrophotometric method for the estimation of sparfloxacin in 0.1 N HCl (gastric simulatory media) as per ICH guidelines. The ?max for sparfloxacin in 0.1 N HCl was found to be 296 nm. The drug follows linearity in-between the concentration range of 2-12?g/ml with a correlation coefficient value of 0.999. The proposed method was applied onto the bulk and marketed pharmaceutical formulation (tablets) so as to find out contents of drug. Estimated drug was found to be in-between 98.43 to 101.57 % in marketed tablets, a good agreement with the claimed level. The accuracy of the method was verified by recovery experiment performed at three different levels- 75%, 100%, and 125%. The % recovery was found to be in the range of 97.31-101.45%. The accuracy and reproducibility of the method was indicated by low values of % RSD. The precision of the method was studied through intraday and Interday variations and repeatability. The % RSD value< 2 confirmed the precision of developed method. Ruggedness of the proposed method was studied with the help of two analysts. The proposed method was found to be a rapid yet successful tool for routine analysis of sparfloxacin in the bulk and in the pharmaceutical dosage forms. Keywords: Fluorquinolone; sparfloxacin, Quantitative determination, UV, validation.

The present work deals with the isolation, identification of the compound from the flowers of Cascabela thevetia. The structure of the isolated compound was elucidated by physical and chemical methods. The isolated compound was characterized using various spectroscopic data such as UV, 1H NMR, 13C NMR, MS.

The objective of this study was to establish a reasonably simple and reliable method to estimate Poly (hexamethylene biguanide) hydrochloride [PHMB] in marketed formulation. New methods like UV & HPTLC were developed for estimation of Poly (hexamethylene biguanide) hydrochloride with all the validation parameters within range. Both the methods were developed and validated as per regulatory guidelines. UV method was developed in Water as a solvent. Linearity was found to be 2-12μg/ml for Poly (hexamethylene biguanide) hydrochloride. The method was found to be accurate, precise according to acceptance criteria. The proposed UV method can be applicable for the estimation of the drug in marketed formulation. HPTLC method was developed using mobile phase Methanol:O-phosphoric acid (10: 0.5 %v/v). In HPTLC method linearity was found 2000-12000ng/spot for Poly (hexamethylene biguanide) hydrochloride. HPTLC method was found to be simple, accurate and precise. The developed HPTLC method can be applicable for the estimation of the drug in marketed formulation. Developed methods can be applied in routine analysis for the estimation of Poly (hexamethylene biguanide) hydrochloride in marketed formulation.