Methylcobalamin(MCA)

A simple, precise, rapid, selective, and economic high-performance thin layer chromatography (HPTLC) method has been established for simultaneous analysis of Citicoline Sodium and Methylcobalamin. HPTLC method was developed using on precoated silica gel F254 G60 plates as stationary phase, using methanol: acetonitrile: water: triethylamine (8.5:1.5:1:0.5 v/v/v) as mobile phase. The plates were scanned at approximately 254 nm for both Citicoline sodium and Methylcobalamin respectively. In HPTLC method both the drugs were resolved using proposed mobile phase and Rf value was found to be 0.39 for Citicoline sodium and Rf 0.61 for Methylcobalamin. The method was found to linear in the range 1000-6000 ng/band for citicoline sodium and methylcobalamin respectively. This HPTLC procedure is economic, sensitive, and less time consuming than other chromatographic procedures. It is important tool for analysis of combined dosage form. Proposed method can be successfully applied for the quantitative determination of Citicoline Sodium and Methylcobalamin in Bulk drug and Pharmaceutical dosage form.