Histological Effect of 5-Aminosalicylic Acid and Vitamin-E on Acrylamide Induced Prostate Toxicity In Rat

The aim of this work was to study the association between subacute acrylamide  exposure and prostate toxicity in male rats; and to compare the effect of two known antioxidants: Vitamin-E and 5- aminosalicylic acid on the induced prostate toxicity in male rats. King Fahad Medical Research Centre, King Abdulaziz University, Jeddah, Saudi Arabia. A total of 49 adult wistar rats (250 ± 20g) of 60 days age were divided into seven groups (control, acrylamide alone, acrylamide + 5-aminosalicylic acid, acrylamide + Vitamin -E, acrylamide + 5-aminosalicylic acid + Vitamin-E, Vitamin-E alone, 5-aminosalicylic acid alone). After 5 days of acrylamide oral gavage, rats were observed for 24 hours and sacrificed. Histopathology for the prostate and testosterone hormone were carried out. No significant changes were observed in testosterone, lactate dehydrogenase serum level and rats’ body weight. Rats treated with 5-aminosalicylic acid alone did not show any protection against acrylamide induced prostate toxicity. Further Vitamin-E alone did not show any protection against acrylamide induced prostate toxicity. Interestingly, injection of acrylamide treated rats with both Vitamin-E and 5-aminosalicylic acid concomitantly showed moderate improvement in the general histology of the prostate toxicity induced by acrylamide. Injection of acrylamide treated rats with 5-aminosalicylic acid or Vitamin-E alone did not show protective effect on acrylamide induced prostate toxicity on the level of prostate histology. However concomitant treatment of acrylamide treated rats with both antioxidants showed moderate improvement in general prostate histological structure.