Inhibition of Formation of Fructose Induced Advanced Glycation End Products by Momordica chochinchinensis

ABSTRACTThe present study evaluates the antioxidant potential of hydro alcoholic pericarp extract of Momordica chochinchinensis (HAPMC) using different model systems. To establish the link between glycation and oxidation processes, the HAPMC extract has been evaluated for its in vitro radical scavenging  activity against superoxide, hydroxyl, hydrogen peroxide, nitric oxide, ferric and ABTS+ radical and antiglycation activities like the inhibitory activities on bovine serum albumin (BSA) and as well as protein oxidation markers including protein carbonyl formation (PCO). The results indicated that the HAPMC possesses the highest antioxidant potential against superoxide, hydroxyl, hydrogen peroxide, nitric oxide, ferric radical and ABTS+ radical. The HAPMC extract at different concentrations (10–250 µg/ml) has significantly quenched the fluorescence intensity of glycated BSA and the glycoxidation measured in terms of advanced glycation end products (AGEs). Furthermore, the study demonstrated that the inhibitory effect of HAPMC extract in preventing oxidative protein damages including effect on PCO formation, which are believed to form under the glycoxidation processes. These results clearly demonstrate that the HAPMC, owning to its antioxidant content, is capable of suppressing the oxidative stress, carbonyl stress and formation of AGEs and protein oxidation in vitro. Keywords: Advanced glycated end products, Oxidation, Momordica chochinchinensis