Analytical Method Development and Validation for the Simultaneous Estimation of Cefixime Trihydrate and Ornidazole in Combined Dosage form by UV-Spectrophotometric Method

A Simple, precise, accurate and economical UV-spectrophotometric method has been developed and validated for simultaneous estimation of Cefixime trihydrate (CEF) and Ornidazole (ORD) in combined dosage form. In simultaneous equation method, CEF and ORD were quantified using their absorptivity values at selected wavelengths 288 nm and 311 nm respectively. The linearity range was found to be 5-50 μg/ml for CEF and 5-50 μg/ml for ORD. Different analytical parameters such as linearity, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ) were determined as per ICH guidelines. Limit of detection and quantification values CEF 1.10 and 3.33 μg/ml and for ORD 1.58 and 4.79 μg/ml respectively. The recovery values between prescribed limit of 98-102% shows that method is free from interference of excipients present in formulation. The developed method was free from interferences due to excipients present in formulation and it can be used for routine quality control analysis.