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American Journal of Pharmacy and Health Research

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UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR QUANTITATIVE ESTIMATION OF SULFAMETHOXAZOLE IN PHARMACEUTICAL DOSAGE FORM

Published in June 2026 Issue 06 (Vol. 14, Issue 6, 2026)

UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR QUANTITATIVE ESTIMATION OF SULFAMETHOXAZOLE IN PHARMACEUTICAL DOSAGE FORM - Issue cover

Abstract

A simple UV–visible spectrophotometric method was developed and validated for the quantitative estimation of sulfamethoxazole in tablet dosage forms in accordance with ICH guidelines (ICH, 2005). The method employs filtered water as solvent and measurement of absorbance at 525 nm, with calibration curves showing good linearity over the concentration range of 5–30 μg mL−1μg mL−1. Absorptivity values were calculated at the selected wavelength. Accuracy, evaluated through recovery studies, showed percent recovery between 98.90 and 99.96% with %RSD values below 2%, confirming the reliability of the method. Precision studies (intra- and inter-day) yielded a %RSD of 0.678, while ruggedness assessment using six replicate absorbance measurements produced a %RSD of 0.253, indicating good robustness. All validation parameters met ICH acceptance criteria, demonstrating that the proposed method is simple, rapid, accurate, precise, and suitable for routine quality control analysis of sulfamethoxazole in pharmaceutical tablet formulations.

References

  1. [1]1. Brunton LL, Hilal-Dandan R, Knollmann BC. Goodman & Gilman’s The Pharmacological Basis of Therapeutics. 14th ed. New York: McGraw-Hill Education; 2023.
  2. [2]2. Katzung BG, Vanderah TW. Basic and Clinical Pharmacology. 16th ed. New York: McGraw-Hill Education; 2021.
  3. [3]3. Sweetman SC, editor. Martindale: The Complete Drug Reference. 40th ed. London: Pharmaceutical Press; 2020.
  4. [4]4. Drug Bank. Sulfamethoxazole. Toronto (ON): Drug Bank; 2024. Available from: https://go.drugbank.com
  5. [5]5. Sohrab i, M.R., Fathabadi and A.H. Nour, Simultaneous Spectrophotometric determination of sulfamethoxazole and trimethoprim in pharmaceutical preparations by using multivariate calibration methods. J. Appl. Che. Res.,3:47-52,2010.
  6. [6]6. Islam, T., S. Ferdous, P. Jain and H.M. Reza, Method development and validation of baclofen mouth dissolving tablets by UV spectroscopy. EUR. J. Applied Sci., 5:7-11: 2013.
  7. [7]7. Bana, T., G. Yadav and R. Dud he. Development and validation of oseltamivir phosphate API by UV-spectrophotometer. Global J. Pharmacology., 7: 294- 297:2013.
  8. [8]8. Wang JC, Zhang Q, Cai DF. Stability indicating validated HPLC method for analysis of berberine hydrochloride and trimethoprim in the pharmaceutical dosage form. J Chemistry 2013:1-9.
  9. [9]9. Shamsa F, Amani L. Determination of sulfamethoxazole and trimethoprime in pharmaceuticals by visible and UV spectrophotometry. Iran J pharm Res 2016: 1:31-6.
  10. [10]10. Skoog DA, Holler FJ, Crouch SR. Principles of instrumental analysis. 6th ed. Belmont: Cengage Learning; 2007.
  11. [11]11. Chatwal GR, Anand SK. Instrumental methods of chemical analysis. 5th ed. Mumbai: Himalaya Publishing House; 2007.
  12. [12]12. Beckett AH, Stenlake JB. Practical pharmaceutical chemistry. 4th ed. New Delhi: CBS Publishers; 2002.
  13. [13]13. Vogel AI. Textbook of quantitative chemical analysis. 6th ed. Harlow: Pearson Education; 2000.
  14. [14]14. Connors KA. A textbook of pharmaceutical analysis. 3rd ed. New York: Wiley-Interscience; 1997.
  15. [15]15. Rang HP, Dale MM, Ritter JM, Flower RJ. Rang and Dale’s pharmacology. 8th ed. London: Elsevier Churchill Livingstone; 2016.

Authors (6)

Dr.Ganesh Akula

Surabhi Dayakar Rao College of...

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Konda Pradeep

Surabhi Dayakar Rao College of...

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Afreen Sulthana

Surabhi Dayakar Rao College of...

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Chikkudu srija

Surabhi Dayakar Rao College of...

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Nayini Shravya

Surabhi Dayakar Rao College of...

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Kesari Miranjipally Indu

Surabhi Dayakar Rao College of...

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Article Information

AJPHR6140003

AJPHR-01-000921

23-31

2026-06-09

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How to Cite

Akula & Pradeep & Sulthana & srija & Shravya & Miranjipally, K. (2026). UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR QUANTITATIVE ESTIMATION OF SULFAMETHOXAZOLE IN PHARMACEUTICAL DOSAGE FORM. American Journal of Pharmacy and Health Research, 14(6), 23-31. https://ajphr.com/articles/AJPHR6140003

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