Ngondi Judith Laure

We investigated the phytochemical content, the antioxidant capacity and the anti-diabetic properties of aqueous and hydroethanolic (50%) extracts of the leaves of Acalypha wilkesiana Muell. Arg. A phytochemical screening was carried out to identify the different bioactive metabolites. The polyphenolic content and the antioxidant capacity of both extracts were determined using Folin-ciocalteu and FRAP (Ferric Reducing Antioxidant Power) methods respectively. In vivo studies were conducted in normal and streptozocin induced diabetic male rats. The effect of both extracts on postprandial blood glucose level was determined in 16 fasted normal rats. The effect of the extracts on fasting blood glucose was determined in streptozotocin induced-diabetic rats (400 mg/kg) for 5 hours. The hydroethanolic extract contained more polyphenols compared to the aqueous extract (218,51 ± 17,41 vs 111,02± 12,15 mg equivalent catechin /g of extract; p

The assessment of nutritional status based on the 2006 WHO (World Health Organisation) Standards versus the 1977 NCHS (National Center for Health Statistics) Reference has revealed some differences. A chief criticism of the use of the 1977 NCHS Reference has been that, because the reference is based on children from a developed country it should not be applied for all children around the world. The 2006 WHO Standards, they are assumed to be more robust in assessing the nutritional status of the children, especially infants, than was the case for the 1977 NCHS Reference. In the present paper, we compare the estimates of wasting, stunting and underweight obtained from the 2006 WHO standard and the 1977 NCHS reference for the Cameroonian. A total of 1481 children under five years were interviewed in a cross sectional survey including 1343 from 6-59 months. The nutrition status of the children was assessed using the 1977 NCHS Reference and the 2006 WHO Standards. The Hong-de Onis algorithm was also applied separately for children in all age groups, stratified by region and by sex. The 2006 WHO standard compared to the 1977 NCHS standard classify more children as underweight and stunted (8.0% vs 6.8% and 41.0% vs 38.1% respectively). Both references classified 56.8% of children as not stunted, 91.2% as not wasted and 69.4% as not underweight.  More children are classified as undernourished using the algorithm compared to directly applying the 2006 WHO Standards except for the 6-11months age group. The assessment of malnutrition using 2006 WHO Standards classify significantly more children as underweight and stunted and is closer to that of a healthy population than the 1977 NCHS Reference.

Several micronutrients are essential for adequate growth of children. However, little information is available on multiple micronutrient status of preschool children in Cameroon. The present study was designed to evaluate the relationship between multiple micronutrient levels and nutritional status among preschool school children. This was a cross-sectional study of 331 children 6-59 months in the North and Far North Regions of Cameroon. Serum concentration of magnesium, calcium, copper, and zinc were measured by inductively coupled plasma mass spectrometer. Ferritin, sTfR, RBP, CRP, AGP were measured by Sandwich ELISA. Weight-for-age, height-for-age and weight-for-height were used to estimate the children's nutritional status. The prevalence of stunting, underweight, wasting among preschool children was 42.0%, 26.0%, and 6.6%, respectively. The mean serum levels of magnesium, calcium, Body Iron Stores, copper,  and zinc were 19.9±0.1 (μg/dl), 80.7±0.3 (μg/dl), 1.02±0.25 (mg/kg), 201.5.30±33.7 (μg/dl), and 49.4±0.8 (μg/dl) respectively. The proportion of low plasma Zinc Concentration was 85.6%, Iron Deficiency anemia was 24.2% with 37.9 % of Iron deficiency associated to anemia. Height-for-age showed significant positive correlation with the levels of Zinc (r=0.093, p