antioxidant

ABSTRACTSpinacia oleracea is a green leafy vegetable widely consumed for its nutritional value. The present research work is focused on spinacia oleracea seed extract for evaluation of in vitro antioxidant and in vivo anxiolytic activities. Spinach leaves antioxidant properties are well documented but the bioactive potential of seeds remains unexplored. Hence this research work aims to explore the antioxidant and anxiolytic effects of Spinacia oleracea seed extract using standard in vitro and in vivo methods. The in vitro antioxidant potential of the spinacia oleracea seed extract at dose of 50,100,300 and 500µg/ml were assayed using hydroxyl radical scavenging, nitric oxide, and DPPH radical scavenging assay and results are compared with standard reference drug Gallic acid at concentration 2.5µg/ml. In behavioral studies, spinacia oleracea seed extract at dose of 75mg/kg and 150mg/kg was tested on albino mice subjected to standard behavioral paradigms such as elevated plus maze, open field test, hole board test, and y-maze. The results of these effects were compared to standard anxiolytic drug diazepam. The results obtained were encouraging and indicated significant antioxidant and anxiolytic effect. The FTIR studies revealed that the seed extract was rich in phenolic compounds known for its antioxidant property.Keywords: Spinacia oleracea; antioxidant; DPPH; anxiolytic; elevated plus maze; FTIR  

ABSTRACTThe objective of the present work is to evaluate and establish the potential anti-inflammatory and antioxidant action of the Resveratrol and BioPerineÒ combination against DPPH was found to be 57.01µg/mL. The Resveratrol and BioPerineÒ combination was able to reduce the inhibition after the third hour significantly (p<0.01) exhibiting 50.87% reduction in edema volume. Keywords: Resveratrol, BioPerine, antioxidant, anti-inflammatory, DPPH, Rat paw edema, carrageenan

ABSTRACTSome herbal remedies are said to have antioxidant activity, reducing oxidative stress in cells and are therefore useful in the treatment of many diseases such as diabetes. This study consisted of the evaluation of the antioxidant activity in vitro and in vivo of an aqueous extract of Vernonia amygdalina (EAVa) in healthy rats and diabetic rats. The antioxidant power of EAVa, evaluated in vitro by assaying the anti-free radical activity by the 2,2-diphenyl-1-picrylhydrazyl test, shows that this extract (IC50 = 26.07 ?g/mL), just like gallic acid (IC50 = 2.13 ?g/mL), reduces this free radical, and therefore has an antioxidant power. The study of antioxidant activity in vivo on rats shows that EAVa, at a dose of 1200 mg/kg BW, strengthens the antioxidant balance in healthy animals and decreases the state of oxidative stress induced by the injection. streptozotocin (STZ). In fact, the injection of STZ disrupts the oxidative defense system in rats. In pathological changes caused by the presence of oxygen radicals, EAVa causes depletion of hepatic glutathione levels and increased levels of liver malondialdehyde, catalase and superoxide dismutase. EAVa thus maintains the balance of the antioxidant balance of liver cells, which allows it to be used against diseases associated with oxidative stress, and therefore the pathological state of diabetes. This antioxidant power of the aqueous extract of Vernonia amygdalina would justify the use of this plant in the prevention and treatment of diabetes and associated pathologies. Keywords: Diabetes, antioxidant, Vernonia amygdalina, hepatic glutathione, catalase, malondialdehyde, superoxide dismutase.

We investigated the phytochemical content, the antioxidant capacity and the anti-diabetic properties of aqueous and hydroethanolic (50%) extracts of the leaves of Acalypha wilkesiana Muell. Arg. A phytochemical screening was carried out to identify the different bioactive metabolites. The polyphenolic content and the antioxidant capacity of both extracts were determined using Folin-ciocalteu and FRAP (Ferric Reducing Antioxidant Power) methods respectively. In vivo studies were conducted in normal and streptozocin induced diabetic male rats. The effect of both extracts on postprandial blood glucose level was determined in 16 fasted normal rats. The effect of the extracts on fasting blood glucose was determined in streptozotocin induced-diabetic rats (400 mg/kg) for 5 hours. The hydroethanolic extract contained more polyphenols compared to the aqueous extract (218,51 ± 17,41 vs 111,02± 12,15 mg equivalent catechin /g of extract; p

Shorearobusta widely distributed in moist and dry forest in the tropics, has been paradoxically described as deciduous, semi-decidous or evergreen species. The resin is astringent sweet acrid, cooling anodyne, vulnerary, antibacterial, deodorant constipating and detergent. It is useful in hyperhydrosis, vitiated conditions of pitta, wound ulcers, pruritis, fractures, fever, diarrhoea, dysentery etc. The main aim of the present study was to carry out the in vitro antioxidant activity of ethanolic extract Shorearobusta oleoresin and GC-MS analysis of the oil obtained from petroleum ether fraction by column chromatography. In vitro antioxidant activity screening was done by oxygen radical scavenging assay such as DPPH and Iron chelating method and the IC50 was found to be 357.44and 151.27µg/mlusing ascorbic acidas standardwith IC50 value of 52.28 and 17.85µg/mlrespectively.Total antioxidant was 94.95µg equivalent to that of ascorbic acid.Thirty chemical constituents have been identified by GC-MS; the major chemical constituents are 7-Tetracyclo [6.2.1.0(3.8)0(3.9)]undecano (7.23%), Bis(2-ethylhexyl) phthalate (7.06%),3-Octenoic acid, methyl(tetramethylene)Si (6.25), Ursa-9(11),12-dien-3-ol (4.96%), phenol (4.68%) and 1-Fluoroforskolin (4.08%).

The aim of present study was to investigate the In-vitro anti-oxidant and In-vivo diuretic activity of Ethyl acetate extract of Nardostachys jatamansi DC roots in rats. The preliminary phytochemical investigation was carried out to identify the various chemical constituents present in Ethyl acetate extract. In-vitro antioxidant activity was measured by using hydrogen peroxide scavenging activity, reducing power assay, DPPH scavenging activity. Ethyl acetate extract of Nardostachys jatamansi DC roots administered in rats at 100mg/kg, 200mg/kg and 500mg/kg, orally and compared with furosemide (20mg/Kg, intraperitoneally) as the standard. The diuretic effect of the extracts was evaluated by measuring urine volume, electrolyte concentration, pH, density, conductivity, saluretic index, diuretic index and carbonic anhydrase inhibition ratio. The results were analyzed by One Way Analysis Of Variance (ANOVA). The results showed that ethyl acetate extract of Nardostachys jatamansi DC roots showed significant Anti-oxidant activity. Extract induced strong diuresis at higher doses and was not accompanied with a reduction in urinary K+ levels. Further, there was no alkalization of urine. Collectively, these observations suggest that the extract is not acting as potassium-sparing diuretics. Extract showed strong diuretic index, saluretic index, natriuretic index and weak carbonic anhydrase inhibition ratio. The findings concluded that Nardostachys jatamansi DC roots extract exhibit anti-oxidant and diuretic activity and further studies are suggested to isolate the active principles responsible for the activity.