anti-inflammatory

ABSTRACTThe present work focused on preparing hydroalcoholic extracts of Mangifera indica and Ocimum sanctum leaves and establishing the anti-inflammatory potential of the combined extracts in experimental model. The extraction yield of the hydroalcoholic mixture was found to be 36.2 % for Mangifera indica and 43.7 % for Ocimum sanctum. The findings of preliminary phytochemical analysis suggest the presence of alkaloids, saponin glycosides, phenolics, terpenoids, and flavonoids in the leaf of the Mangifera indica while alkaloids, sterols and glycosides were not present in Ocimum sanctum. The total phenolic content of the extracts of M. indica and O. sanctum were 26.86 ± 0.308 and 37.56 ± 0.665 GAE mg/g, respectively. The phenolic content was highest in the combined extract (CE, 1:2) of all the three combinations with total phenolics 81.53 ± 1.855 GAE mg/g. The extracts were individually and in combination (1:1, 1:2 & 2:1) subjected to determination of anti-inflammatory potential using carrageenan induced rat paw edema model. Ibuprofen at dose of 10 mg/Kg inhibited 69.23% edema after 4h of administration whereas the maximum edema inhibition exhibited by the combined extracts was 52.47% (MIE:OSE, 1:2)  at the end of 4h. The anti-inflammatory action was consistent with the phenolic content of the extracts; with a higher amount of OSE in the mixture presenting a better inhibition of paw-edema. Keywords: Mangifera indica, Ocimum sanctum, paw edema, anti-inflammatory, phenolics

ABSTRACTThe objective of the present work is to evaluate and establish the potential anti-inflammatory and antioxidant action of the Resveratrol and BioPerineÒ combination against DPPH was found to be 57.01µg/mL. The Resveratrol and BioPerineÒ combination was able to reduce the inhibition after the third hour significantly (p<0.01) exhibiting 50.87% reduction in edema volume. Keywords: Resveratrol, BioPerine, antioxidant, anti-inflammatory, DPPH, Rat paw edema, carrageenan

The present study aims on assessing the anti arthritic activity using bovine serum albumin and anti-inflammatory activity using human red blood cell membrane stabilization model. Citrus limetta, commonly known as sweet lime, belongs to family Rutaceae. It is a rich source of flavanoid, d-limonene, alkaloids, steroids, volatile oils and polyphenols. Various extracts of sweet lime peel at different concentrations (50, 100, 200, 400 and 800µg/ml) showed the significant ameliorating effect of hypotonic saline induced RBC membrane damage when compared against standard drug aspirin whereas ameliorating effect by inhibiting protein denaturation on bovine serum albumin in vitro model against standard drug diclofenac sodium. Protective effect on heat induced protein denaturation can be used as a tool to study the in vitro anti-arthritic activity whereas protective effect on heat and hypotonic saline-induced erythrocyte lysis is known to be a very good index of anti-inflammatory activity of any agent. The results of the present study revealed that at various concentrations of Citrus limetta peel extracts when subjected to in vitro anti-arthritic and anti-inflammatory activity showed % inhibition to be significant when compared with standard drug. Thus Citrus limetta fruit peel demonstrates a potential anti-inflammatory and anti-arthritic effect which may be attributed to its anti-oxidant property.

The inflammation is a complex process, which is frequently associated with pain and involves several events. Anti-inflammatory compounds can act on many steps of pathophysiological process by blocking the biosynthesis of proinflammatory mediators; by direct interaction with a key enzyme or by decreasing enzyme expression. The pomegranate, (Punica granatum L.)  has been used extensively in the folk medicine for a number of therapeutic purposes. In the present investigation antiinflammatory activity of pomegranate whole fruit (EPWF) and seeds (EPS) ethanol extracts and synthetic ellagic acid(EA) was evaluated in swiss albino mice using ethylphenylpropiolate and carrageenan induced edema models. EA and EPWF exhibited inhibitory effect (P