Ocimum sanctum

ABSTRACTThe present work focused on preparing hydroalcoholic extracts of Mangifera indica and Ocimum sanctum leaves and establishing the anti-inflammatory potential of the combined extracts in experimental model. The extraction yield of the hydroalcoholic mixture was found to be 36.2 % for Mangifera indica and 43.7 % for Ocimum sanctum. The findings of preliminary phytochemical analysis suggest the presence of alkaloids, saponin glycosides, phenolics, terpenoids, and flavonoids in the leaf of the Mangifera indica while alkaloids, sterols and glycosides were not present in Ocimum sanctum. The total phenolic content of the extracts of M. indica and O. sanctum were 26.86 ± 0.308 and 37.56 ± 0.665 GAE mg/g, respectively. The phenolic content was highest in the combined extract (CE, 1:2) of all the three combinations with total phenolics 81.53 ± 1.855 GAE mg/g. The extracts were individually and in combination (1:1, 1:2 & 2:1) subjected to determination of anti-inflammatory potential using carrageenan induced rat paw edema model. Ibuprofen at dose of 10 mg/Kg inhibited 69.23% edema after 4h of administration whereas the maximum edema inhibition exhibited by the combined extracts was 52.47% (MIE:OSE, 1:2)  at the end of 4h. The anti-inflammatory action was consistent with the phenolic content of the extracts; with a higher amount of OSE in the mixture presenting a better inhibition of paw-edema. Keywords: Mangifera indica, Ocimum sanctum, paw edema, anti-inflammatory, phenolics

ABSTRACTThis review paper purpose is the ‘Queen of herb’’ means holy basil/Tulsi is how to showing it Antimicrobial activity by using different type of method. This paper also study the pharmaceutical used of Tulsi & its Pharmacological activity of Tulsi like. Antimicrobial activity, Antifungal activity, Anti-inflammatory& Analgesic activity. It is main symbol of the Hindu region tradition this is plant having unique odor found in the semitropical and tropical country. In the world. This is plant mainly used for reducing the cough. Holy basil is ancient Hindu scriptures, Tulsi occupies the supreme position among the herb so that it referred to as “MOTHER”. It has been used Ayurveda and siddha practices for it’s supposed treatment of diseases. It having medicinal her that belong to the mint family and is found in 150 different worldwide. Tulsi has been used in thousands of years in Ayurveda for its diverse healing properties special about this plant is apart from being considered holy Tulsi is also reserved as great healer Keyword: Tulsi, Holy basil, Ocium tenuiflorum, Ocimum sanctum, Eugenol

Photoaging is the term used to describe the characteristic changes that appear in the skin when it is chronically exposed to Ultra Violet (UV) rays. Photoaging is associated with increased risk of causing skin cancer. Antioxidants play an important role in prevention and treatment of photoaging. To evaluate the preventive efficacy of oral Ocimum sanctum against photoaging in mice model. Six weeks old Swiss Albino mice of either sex weighing 25g to 35g were divided into three groups A, B, C (n=6). Group A was given UV irradiation alone, group B was given UV irradiation and oral Ocimum sanctum, group C was neither given UV irradiation nor oral Ocimum sanctum, for 8 weeks. Groups A, B and C were sacrificed and evaluated for moisture content, epidermal thickness by histopathology and antioxidant property by TBARS assay. Results were expressed as Mean ± SEM. Statistical analysis were done by One way ANOVA and student’s unpaired t test. Efficacy in prevention: Moisture content of group A (11.57±0. 29) showed significantlylower value than group B (21.96 ±2. 1). Group B has a lower value compared to group C (27.56 ±2. 4). On TBARS assay, Group A (1.083±0.03) value is more than group B (0.459±0.01), Group B value is more than group C (0.536±0.01). On histopathology examination group A showed atrophy of epidermis. B showed near normal histology and C showed normal histology. This study has proved that oral Ocimum sanctum can be used for prevention of photoaging. It warrants further study in humans before including them in the prophylaxis of photoaging.