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The Harbone method of extraction was used in the extraction of Urena Lobata roots. The crude extracts were separated using TLC. Result of the phytochemical screening showed the presence of saponins, cardiac glycosides, and tannins. The pure chloroform extract of the roots of Urena lobata and that of ethyl acetate were analyzed using spectroscopic instruments; Fourier Transform Infra-red(FTIR), UV-Visible, Nuclear Magnetic Resonance (NMR), and GC-MS. Combination of the results from these analysis suggested the compounds Phathalic acid, dibutylglycolate (C18H24O6) for the  chloroform extract and  1,2-Benzenedicarboxylic acid, 2-methylpropyl, 3- methylbutyl ester (C17H24O4 ) for ethyl acetate extract as the active compounds. These compounds are phthalates which are known to have antimicrobial properties. Antimicrobial analysis carried out on the extracts and the chloroform extract showed very high sensitivity to the test organisms: Aspergillus flavus, Aspergillus niger, Candida albican; S. aureus, E. coli, Enterobacter aerogenes, Proteus vulgaris, Streptococcus species, Bacillus species, Pseudomonas pyocyania and Klebsiella aerogenes.

Phytochemical techniques played a significant role in searching raw materials and resources for pharmaceutical industry. Preliminary Phytochemical tests are helpful in finding and locating chemical constituents which are source of pharmacologically active principles.  were carried out by employing standard methods for conducting Qualitative phytochemical analysis for studying the presence of active compounds like alkaloids, glycosides, phenols, saponins, tannins flavonoids, volatile oils, reducing sugars and steroids. Aqueous pericarp extract of Litchi chinensis showed presence of phenolic compounds, except flavonoids, volatile oils alkaloids, glycosides, saponins, tannins, reducing sugars and steroids. The antimicrobial activity of aqueous pericarp extract of Litchi chinensis was studied by agar well diffusion method in vitro.   The effect of antimicrobial potential was examined Salmonella typhi, Vibrio cholerae, Shigella dysenteriae, Enterococcus faecalis, Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus and Candida albicans. The aqueous extract of the fruit pericarp has showed consistently significant inhibitory activity on different bacterial species tested and found the significance of antimicrobial activity of Litchi chinensis.