Second derivative spectrum

Two simple, accurate and reproducible first and second derivative spectrophotometric methods have been developed for the determination of alfuzosin in pharmaceutical formulation. The solutions of standard and sample were prepared in methanol. For the first method, UV spectrophotometry, the quantitative determination of the drug was carried at 245 nm and the linearity range was found to be 1-5 µg/ml. For the first and second derivative spectrophotometric methods the drug was determined at 237 nm and 245 nm. The calibration graphs constructed at their wavelength of determination were found to be linear for UV and derivative spectrophotometric methods. The proposed methods have been extensively validated as per ICH guidelines. The described methods can be readily utilized for the analysis of pharmaceutical formulation. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. Developed spectrophotometric methods in this study are simple, accurate, and precise.