Spectrophotometric method

ABSTRACTA simple, sensitive and rapid spectrophotometric method has been developed for the estimation of Amphetamine (AM) in bulk and in pharmaceutical formulations using 2,3-dichloro-5,6-dicyano-p-benzoquinone (DDQ). The proposed method is based on the formation of charge-transfer complex between AM and DDQ. AM in the presence of DDQ formed pale wine red color complex which showed a maximum absorbance at 465 nm against the blank. The limit of detection and quantitation were 0.1395 mg/ml and 0.4644 mg/ml respectively. The results of analysis were validated by recovery studies, accuracy, precision, LOD, LOQ, robustness and ruggedness, which indicated that the present method can be successfully applied for the determination of AM in pure and in pharmaceutical formulations. Keywords: Spectrophotometric method, Amphetamine, Charge-transfer complex, DDQ.

ABSTRACTA simple and sensitive spectrophotometric method has been developed for the determination of procainamide (PA) in pure and pharmaceutical formulations. The proposed method is based on the formation of charge-transfer complex between the drug and 1-chloro-2, 4-dinitrobenzene (CDNB). PA in the presence of CDNB formed yellowish red colored complex, which showed a maximum absorbance at 410 nm. The limit of detection and quantitation were 0.1183 mg/ml and 0.3940 mg/ml respectively. The influence of commonly used excipients on the determination of PA was studied. Keywords: Spectrophotometric method, Procainamide, CDNB, Charge-transfer complex.