inhibition of protein denaturation

ABSTRACTRheumatoid arthritis (RA) is an evolving, enduring, and a crippling disorder categorized by puffiness, agony, and synovial joints arduousness. The exact reason of this devastating illness is mysterious. However, it is strongly linked to self-protection reaction triggered by various genetic and external factors. The current research work was designed to explore the anti-arthritic probable of the plant Erythroxylum monogynum. The plant leaves was collected, shade dried, blended and extracted (continuous hot percolation) with Hydro-alcoholic solvent. Preliminary phytochemical evaluation were carried out. Entirely the in-vitro models i.e. inhibition of protein denaturation, membrane stabilization and proteinase inhibition were carried out by using standard reference drug diclofenac sodium. In the present study there was significant (p<0.05) inhibition of protein denaturation in both standard and extract treated group and % inhibition of protein denaturation produced by extract at concentrations 100 and 300?g/ml exhibited improved activity than standard (Diclofenac sodium). From the results of the present study it can be stated that Erythroxylum monogynum may control the production of auto-antigens by preventing in-vivo denaturation of proteins in rheumatic diseases. Dose based and significant (p<0.05)of anti arthritic activity in in-vitro designs were found. The results disclose hopeful anti arthritic probables of the herb. However further pharmacological investigation using isolated active ingredients can be carried out to confirm its usefulness and effectiveness. Keywords: Anti-arthritic activity, Erythroxylum monogynum, in-vitro studies, inhibition of protein denaturation, membrane stabilization, proteinase inhibition,