Evaluation of Antibacterial and Antimutagenic Potential of Acokanthera oppositifolia and Leucaena leucocephala

Human beings have used plants for the treatment of diverse ailments for thousands of years. Plant derived chemicals are detoxify carcinogens therefore, the present study was undertaken to evaluate antibacterial and antimutagenic potential of A.oppositifolia and L.leucocephala. Three different solvent extracts of both plants were used to screen antimicrobial activity against two human pathogenic bacteria: E.coli (gram negative) and B.subtilis (gram positive) by disk diffusion assay and MIC also determined by using microdilution assay and antimutagenic potential screened by employing AMESassay against direct acting mutagen Etbr with S. typhimurium TA100 and TA98 strains.The range of antibacterial activity in A. oppositifolia varied from 17±0.73mm to 25±0.8mmagainst E.coli and 18±0.16 to 24±0.14 mm zone of inhibition against B. subtiliswhile, this ranged varied from 12±0.21to 27±0.47mm against E. coli.and19±0.5 to 25±0.26 mm against B. subtilis in L. leucocephala. The antimutagenic range of inhibition percentage varied in A. oppositifolia from 57.97% to 98.93% with TA98 and 84.67% to 99.65 with TA 100. In L. leucocephala the range varied from 5.85% to79.78% with TA98 and 93.93% to 99.54% with TA100. Among all extracts chloroform& acetone extract of A.oppositifoliaandmethanol & acetone extract of L. leucocephalahas got highest antimutagenic and antibacterial potential. Finding of the results we reported that, all the extracts possess significant antibacterial and antimutagenic activityso these plants can be used in the treatment of variety of ailments.