Mohammed Amood AL-Kamarany

Diabetic Milletus(DM).is the most frequent endogenous cause of fat metabolism- disorder. Therefore, the study aims to monitor the lipid profile  in  DM type 2 patients controlled and uncontrolled in Sana’a , Yemen. The study included 150 subjects from major public hospitals in Sana'a city, Yemen. 50 were diagnosed with DM type 2 controlled as group I and 50 were diagnosed with DM type 2 uncontrolled as group II. 50 subjects were healthy volunteers as control group. The biochemical parameters namely Fasting Blood Sugar (FBG) , and Glycelated Hemoglobin (HBA1c) were assayed in three groups. Data obtained were analyzed using suitable statistical tools. The lipid profile namely total cholesterol  mg/dl , triglyceride mg/dl HDL mg/dl, and LDL mg/ were assayed in DM type 2   controlled and uncontrolled and compared with control healthy group. The results were  described namely (166 ± 31 mg/dl ; 196 ± 50 ; 131 ± 28 ) , (146± 60 mg/dl ; 193 ± 94 ; 92 ± 30 ) , (37± 8.2 mg/dl , 36 ± 14, 42 ± 12 ), and (80 ± 25 mg/dl , 95 ± 30 , 76 ± 25) respectively.   According to above results , non different significant (p > 0.05) in all parameters of lipid profile was observed between DM  type 2  controlled and uncontrolled groups . while the results were observed different significant (p < 0.05) in serum levels of total cholesterol , triglyceride and LDL between controlled and healthy group and also non - different significant (p > 0.05) in serum levels of HDL between both . In addition, different significant (p > 0.05) in all parameters of lipid profile was observed between uncontrolled and control healthy groups .The study outcome concluded  that the hyperlipidemia may progressive in DM type 2 patients uncontrolled.

To assess the efficacy of therapeutic on Rotavirus diarrheal hospitalizations among children < 5 years of age   based on world health organization (WHO) guideline. Based on a longitudinal observational study, 1027 fecal samples were collected from children (less than 5 years), suffering from diarrhea attended at the Yemeni Swedish hospital (YSH) in Taiz , Yemen from January 2009  to December 2012 .  Rotavirus infection was detected by re – validated enzyme linkage immunosorbent assay (ELISA) on stool specimens of children. The treatment course consists of  two methods, namely, intravenous rehydration fluid therapy  (IV) for inpatient and oral rehydration fluid  therapy (OR) for outpatient with treatment of the major  symptoms, namely,  fever and vomiting based on anti – pyretic and anti – emetic if necessary . The efficacy of therapy quality outcomes was assessed clinically and reported. Firstly, the results of re - validated  ELISA method were precise to each analyte with percent relative standard deviation (RSD %) of intra-assay and inter – assay (< 5.0 %). Furthermore, the interval of accuracy for the method exhibited well recovery value of 93 - 100 % and the coefficient correlation (R2) value was  0.99 as a good linear method for Rotavirus infection. Secondly ,  A total of  581  out of  1027  (56.57 %) patients were admitted as inpatients for IV  fluid  therapy  ,  while  446  (43.43 %) were seen in the outpatient ward  receiving  OR  fluid  therapy . The recovery of patients was 98.10 % for IV and 98.43 % for OR, statistically, that was not significantly different (p> 0.05) . In conclusion , A successful   Rotavirus  treatment guidelines in Yemen will rely upon best sustained diagnosis by application Good Laboratory Practice (GLP)  which is clear in specific  – precise , reliable and  accurate method to detect the virus  and the best efficacy of therapy by Good Clinical Practice (GCP) which is clear in treatment  of  Rotavirus diarrhea which protects against dehydration by fluid therapy .